- Baroness – Defeat Baroness
- Destro – Defeat Destro
- Firefly – Defeat Firefly
- Strike Lieutenant – Defeat every wave in one Satellite Strike mini -game
- Hack ’n Slash – Finish a level using only melee attacks on ground troops
- Sloppy Joe – Achieve the worst rank on a level without going Man Down
- Man Up – Complete a Jungle or Tundra mission without going Man Down
- Half the Battle – Collect 50% of all File Cards
- Bronze – Collect all File Cards
- Classified – Collect all Intel Cards
- Lost & Found – Collect all GI JOE Contacts
- Triplets – Pick up all thee types of Point Multipliers in one mission
- Arctic Completed – Complete all Arctic missions
- Yo Joe! – Purchase two GI JOE contacts
- Combat Rock – Complete a mission using only Combat class characters
- Go Commando – Complete a mission using only Commando class characters
- Bevvy o’ Heavy – Complete a mission using only Heavy class characters
- The Tables Have Turned – Complete any Arctic, Desert, or Jungle mission using any COBRA character
- Lovers’ Quarrel – Complete any mission using Baroness and Duke
- Arishikage Attack – Complete any mission using Snake Eyes and Storm Shadow
- Girls Night Out – Complete any mission using female chracters only
- Gimme The Keys – Drive every GI JOE vehicle in the game
- Code Cracker – Gain access to a Combat Soldier Class Ability door
- Nimble Access – Gain access to a Commando Class Ability door
- Lost Keys – Gain access to a Heavy Class Ability door
- Turret Syndrome – Operate the turret in every vehicle in the game
- Double Barrel Roll – Perform 10 barrel rolls in unison on any level in co-op
- Higher Score – Replay a mission and earn a higher score
- It Takes Two – Complete any mission in 2 player co-op
- Side Mission Completist – Complete all Sub-operation Missions
- Launch Money – Launch 50 enemies into the air and kill them
- Storm Shattered – Defeat Storm Shadow in all three secret arenas
- Strike Commander – Defeat every wave in all Satellite Strike mini-games
- Battle Master – Collect and spend all 130 possible Battle Points
- No “I” in Team – Complete mission with higher than 50% Dual Targeting kills
- Desert Completed – Complete all Desert missions
- Jungle Completed – Complete all Jungle missions
- Hardcore GI JOE – Complete every Mission on Hardcore Difficulty
- Acceleration Celebration – Activate the Accelerator Suit three times in any mission
- Accomplished Mission – Gain the highest Mission Ranking
- M.A.S.S. Destruction – Destroy the M.A.S.S. Device
- COBRAAAA!!! – Purchase all COBRA characters
- All GI JOE Contacts – Purchase all GI JOE Contacts
- GI JOE – GI JOE